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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUR19N

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5500
Notes
Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions & facilitates the rescue in E. coli of plasmids where dimerization has occurred. The order of the major features in this plasmid is: ura4 - ars1 - SfiI/MCS/HindIII/lacZ' - pMB1 ori - ampR. Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; HindIII--5.5; PstI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, Schizosaccharomyces pombe, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77300
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified