Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUR18N
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5500
- Notes
- Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation. The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions & facilitates the rescue in E. coli of plasmids where dimerization has occurred. The order of the major features in this plasmid is: ars1 - ura4 - SfiI/MCS/EcoRI/lacZ' - pMB1 ori - ampR. Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, Schizosaccharomyces pombe, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77299
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified