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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUN100

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
6200
Notes
YC-type shuttle vector useful for the sectoring-shuffle mutagenesis assay. Also permits visual detection of recombinants by lacZalpha complementation. The order of the major features in this plasmid is: ampR - pMB1 ori - lacZ'/MCS - CEN4 - ARS1 - LEU2. Restriction digests of the clone give the following sizes (kb): EcoRI--3.1 (doublet); SacI--6.2; HindIII--6.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77270
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified