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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUMSV0CAT

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5500
Notes
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7. (ATCC staff) A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi. The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells. [1] A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, E.coli TB1. Related vectors: pSV0-cat, UMS. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37651
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified