Skip to main content
Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUC830

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
2710
Notes
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. [1] pUC830 (ATCC 77218) and pUC931 (ATCC 77219) differ in the orientation of the polylinker. [1] E.coli transformed with this plasmid produce larger colonies when an insert is present in the polylinker than when there is no insert. (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; NheI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. Related vectors: pUC8, M13tg130, bGal130, pUC931. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77218
GenBank
L09142
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence