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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUC-DIS

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
2699
Notes
Created by Moore, July 1995, under contract with NCBI. Vector with a MCS containing only relatively rare enzyme cutting sites flanked by SfiI sites to facilitate removal of the insert. By reducing the number of sites in the plasmid, more sites in an insert can be used for gene disruption experiments. The order of the major features in this plasmid is: SfiI/SacI/MCS/SfiI/lacZ' - pMB1 ori - ampR. Restriction digests of the clone give the following sizes (kb): SphI--2.7; SacI--2.7; KpnI--2.7; SalI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, E.coli. Related vectors: pUC18. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77302
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence