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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pUB110cop1

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4500
Notes
Low copy number shuttle vector. [3] Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130. [3] The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural & segregational stability. [3] Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5. (ATCC staff) Medium is -1 1065 plus kanamycin/neomycin (3 ug/ml). Hosts: Bacillus subtilis YB886, Bacillus, Staphylococcus aureus. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77133
GenBank
M62963
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified