Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUAST
Information
- Plasmid Type
- Insect Expression, Drosophila
- Cloning Method
- Unknown
- Size
- 8904
- Bacterial Resistance
- Ampicillin
- Notes
- Brand and Perimon (1993) Development 118 401-415 pUAST consists of five tandemly arrayed optimized GAL4 binding sites followed by the hsp70 TATA box and transcriptional start , a polylinker containing unique restriction sites for EcoRI, BglII, NotI, Xho, KpnI and XbaI and the SV40 small T intron and polyadenylation site. These features are included in a P-element vector (pCaSpeR3)containing the P element ends (P3' and P5') and the white gene which acts as a marker for successful incorporation into the Drosophila genome. More information: Brand and Perimon (1993) Development 118 401-415 P element-based vector for Gal4-regulated expression of genes in Drosophila.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified