Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pTYB12
Information
- Source/Vendor
- New England Biolabs
- Plasmid Type
- Bacterial Expression
- Cloning Method
- Unknown
- Size
- 7417
- 5' Sequencing 1 Primer
- T7
- Tag 1
- Intein
- Bacterial Resistance
- Ampicillin
- Notes
- pTYB12 is an E. coli cloning and expression vector (7417 bp) used in the IMPACT Protein Purification System which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag. It is a N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker, downstream of the intein tag (the Sce VMA intein/chitin domain, 55 kDa). This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. This vector can be used in conjuction with pTYB2 (NEB #N6702S) to test which fusion construction (N-terminal or C-terminal) maximizes the expression and yield of a target protein.
- Catalog Number
- N6902S
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified