Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pTKbeta
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7481
- Notes
- ATCC size is 7600 bp. The order of the major features in this plasmid is: pUC19 - EcoRI - HSV thymidine kinase promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Designed to express low levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Restriction digest of the clone give the following sizes (kb): EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5,2.7,0.9; NotI--4.4, 3.2; BamHI--3.8 (doublet). (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, E.coli, monkey COS cells, vertebrate cells. Related vectors: pBR322, pADbeta, pNASSbeta, pCMVbeta, pSVbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77178
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified