Skip to main content
Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pTC181

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
2700
Notes
Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.15; NcoI--2.7; PvuII--2.3, 0.40. (ATCC staff) Expression vector designed for converting restriction sites at the end of inserts. Also permits insert detection by lacZalpha complementation and sequencing from M13 universal and reverse primers. Constructed from pUC18 by modification of the multiple cloning site to include some unique cloning sites surrounded by some direct repeat sites. Pairs of identical restriction sites are in the same reading frame to maintain lacZ' insert detection regardless of the enzyme used. [1] The plasmid contains the following restriction sites (approximate kb from nt 1): BglI--0.24, 1.85; NcoI--0.45; PvuI--0.28, 2.10; PvuII--0.31, 0.67. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Talavera A.L. Hosts: E.coli, E.coli HB101. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
87067
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified