Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pTAG-1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6000
- Notes
- Deposited by: Judith L. Fridovich-Keil The order of the major features in this plasmid is: SalI - SV40 polyadenylation trimer - XhoI/MCS/HindIII - beta-globin - PstI - SphI - ApaI - ampR/pGEM-7Zf(+). [1] Contains a trimer cassette of the SV40 major late polyadenylation signal to block background readthrough expression of the reporter gene. [1] A promoter/enhancer-cloning shuttle vector using human beta-globin as the reporter gene. Primarily useful for analysis of gene expression at the RNA level. [1] The ApaI site can be used for cloning enhancer elements. [1] Restriction digests of the clone give the following sizes (kb): PstI--3.8, 2.2; SphI--3.8, 2.2; HindIII--6.0; XhoI--6.0; BamHI--5.5, 0.55. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, vertebrate cells. Related vectors: pbetaGlo4pA from pbetaGlo, from pbetaGloXH from pGEM7, & pJFCAT1, pBLCAT3. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77318
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified