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Vector Database


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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pT

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5423
Notes
Deposited by: J. Justin McCormick The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation. [1] Expression vector containing two multiple cloning sites: the first interrupting the lacZ' gene, and the second being flanked by a murine metallothionein I promoter and splicing and polyadenylation signals. [1] The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2894; SacI--1593; StyI--1627; ClaI--1814; XhoI--1836; BglII--1859; SphI--2730; NsiI--2732; PvuI--2774, 4564; HindIII--2945; DrdI--3406, 5277; BsaI--4264. (personal communication) The order of the major features in this plasmid is: NotI - 5' metallothionein promoter 3' - ClaI/MCSII'/BglII - poly(A) - SphI - 3'lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--3.6, 1.8; EcoRI--2.9, 2.5; HindIII--5.4; BglII--5.4; ClaI--5.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM105, E.coli JM109, vertebrate cells. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77384
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified