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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pSVbeta

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
6894
Notes
Recommended ampicillin concentration is 200 ug/ml. (personal communication) The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - HindIII - pUC19. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining or fluorometric assay. [1] Designed to express high levels of beta-galactosidase or an alternative gene product in COS cells. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6 (doublet); BamHI--3.6, 3.2, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, E.coli, monkey COS-1 or COS-7 cells, vertebrate cells. Related vectors: pBR322, pADbeta, pNASSbeta, pCMVbeta, pTKbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77176
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence