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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pSV2neo

Information

Source/Vendor
ATCC
Alt Name
pSV2 neo
Plasmid Type
Mammalian Expression
Cloning Method
Unknown
Size
5729
5' Sequencing 1 Primer
SV40pro-F
5' Sequencing 1 Primer Sequence
TATTTATGCAGAGGCCGAGG
Bacterial Resistance
Ampicillin
Selectable Marker
Neomycin
Notes
ATCC size is 5600 bp. Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines. (ATCC staff) Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet). (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli, human fibroblast or lymphoblast cells, vertebrate cells. Related vectors: pBR322, SV40, Tn5. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
Catalog Number
37149
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence