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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pSP65CS

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
3080
Notes
pSP64CS (ATCC 37635) and pSP65CS (ATCC 37636) differ only in the orientation of the polylinker relative to the SP6 promoter. [3] The 80 bp EcoRI/HindIII fragment (ends filled) of pGV403 with sequencing configuration was cloned in the SmaI site of modified pSP65 (Tth111 site removed). SP6 promoter is next to EcoRI and directs transcription across the MCS toward HindIII. [3] High copy number plasmid vector for directional sequencing of fragments with a sticky end corresponding to one the polylinker sites or bidirectional sequencing of blunt-ended fragments. Ideal for sequencing nested sets of deletions. [3] Restriction digests of the clone give the following sizes (kb): HindIII--3.2; BamHI--3.2; EcoRI--3.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli JM101. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37636
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified