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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pSE640

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9000
Notes
YC-type shuttle enhancer-cloning vector using beta-galactosidase activity as the reporter. (personal communication) The order of the major features in this plasmid is: MCS - CYC - lacZ - pMB1 ori - ampR - URA3 - ARS1 - SUP11 - CEN4. (personal communication) The UAS has been deleted from the CYC1 promoter, so expression of a CYC1-lacZ fusion protein depends on UAS elements cloned into polylinker region. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; XhoI--9.2; SalI--9.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77272
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified