Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pRL126
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6230
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.3; EcoRI--6.2. (ATCC staff) RL724 (ATCC 37683) and RL726 (ATCC 37684) differ in having the M13 intergenic region in opposite orientations in a different size fragment. This plasmid was constructed from the galK leader of pKO100 fused to the lacZ of pMC1403; the M13 intergenic region of pZ152; the multiple cloning sites of M13mp19; and the trp a terminator of pRL118. This is a promoter-cloning vector with beta-galactosidase reporter gene, trp a terminator upstream of MCS,galK leader with stop codons in 3 reading frames, and intergenic region of M13 to permit ssDNA preparation for mutagenesis and/or sequencing. Medium is 1227 LB plus ampicillin. NCBI gi: 209562 Hosts: E.coli JPA101, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37684
- GenBank
- M20915
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified