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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pRFS10

Information

Plasmid Type
Unspecified
Cloning Method
Unknown
Notes
Hypersynthesis of araC protein has been accomplished by deleting some of the 5' noncoding DNA in front of the araC gene and inserting a 95 bp lac fragment containing the promoter and the ribosome binding site. The level of the araC protein was increased 5000 times over that in the wild type gene. Hypersynthesis, however, does not require the lac ribosome binding site. Apparently a sequence already present in front of the araC gene 'ggag' (base pairs 180-183) can serve as an efficient binding site. Hosts: E.coli. Related vectors: pBR322, paraC116, pRFS11. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified