Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pRB1
Information
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Notes
- In the absence of the products of nifA, ntrA, or both the transcription start site of the nifH/lacZ gene, with a point mutation in the promoter region, is shifted from the wild-type nifH mRNA start site at position 101 to positions 60-62. However, when the products of nifA and ntrA are both present transcription starting at positions 60-62 is abolished and the normal transcription start site at position 101 is used. In wild type plasmid the upstream start sites at 60-62 are also used in the absence of the nifA and ntrA products. [1] proposes that the point mutation in the nifH/lacZ promoter region in pRB1 may activate a pre-existing, low-efficiency promoter sequence. NCBI gi: 208904 Hosts: E.coli, Klebsiella pneumoniae. Related vectors: Klebsiella pneumoniae. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- GenBank
- M13381
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified