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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pRAN4

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
11400
Notes
Homologous recombination between pRAN4 and a clone in pYAC4 results in replacement of sequences in the right arm of pYAC4 with those of pRAN4 encoding ampR, G418R, and ADE2. The 1.8 kb BamHI-EcoRI fragment of pRAN4 is homologous to the SmaI-ApaI fragment of pYAC4. Transform S. cerevisiae containing YAC clones with BamHI-linearized pRAN4 and select for recombinants that grow in the absence of adenine (Ura- Ade+ Trp+). Maintenance of the E. coli ori, ampR and kanR genes allows plasmid rescue of YAC insert ends. Restriction sites available for right-end plasmid rescue include XhoI, XbaI, BglII and HindIII. The order of the major features of the plasmid is: BamHI - 1.8 kb pYAC4 homologous sequence - EcoRI - pMB1 ori/ampR - HindIII - SV40 ori - BglII - neo (kanR, G418R) - SmaI - ADE2 - EcoRI - HindIII - Tetrahymena telomere. [1] Modification shuttle vector to construct larger yeast artificial chromosomes (YACs) by mitotic recombination. Also facilitates transfer to mammalian cells. [2] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: D.C.Markie Hosts: Saccharomyces cerevisiae, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77481
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified