Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pQF110
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8800
- Notes
- Promoter-cloning shuttle vector permitting visual detection of activity from divergent promoters. Uses the promoterless luciferase gene from Vibrio harveyi (luxAB) and the promoterless alkaline phosphatase (phoA) as the reporter groups. The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - phoA - Shine-Dalgarno sequence (SD) - translational stop codons - multiple cloning site - tsc - SD - luxAB. phoA and luxAB are oriented divergently, with the HindIII end of the MCS closer to luxAB. Restriction digests of the clone give the following sizes (kb): PstI--5.6, 3.2; HindIII--8.8; BamHI--8.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM109, broad host range, E.coli, Pseudomonas aeruginosa PAO1, E.coli K-12. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77113
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified