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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pPROK-1

Information

Source/Vendor
Clontech
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4600
Notes
Hosts: E.coli JM109. Related vectors: pBR322. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.) pPROK-1 is a prokaryotic expression vector using tac as a strong promoter. In a lacIq bacterial host (i.e. JM109), the tac promoter is repressed but it may be derepressed by IPTG. Other features of this vector include: 1) a MCS with unique restriction sites EcoRI, NcoI, SmaI, HindIII for cloning and 2) a strong rrB ribosomal RNA transcription terminator, T1T2 to prevent overexpression of RNA species.
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified