Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pPH3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 2500
- Notes
- Expression is repressed in the presence of excess inorganic phosphate (>1%) and high concentrations of glucose (>1%). Maximal induction was observed at limiting inorganic phosphate (<0.1 mM) and normal glucose (0.2-0.4%). The order of the major features in this plasmid is:L1 terminator - ugp promoter - EcoRI - MCS - HindIII - rpoC terminator - ori - bla. The nucleotide sequence of the transcription start point before the MCS is GCGAGCATAA AACGCGT. An expression vector useful in possibly all E.coli strains containing a functional pho regulatory circuit. The insert must supply the ribosome-binding site. Restriction digests of the clone give the following sizes (kb): HindIII--2.6; EcoRI--2.6; HindIII/EcoRI--2.6; BglII--1.8,0.80. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli, E.coli DH5alphaF'. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37843
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified