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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pPB101

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
12280
Notes
A plasmid expression vector constructed from spleen necrosis virus, an avian retrovirus. [2] The insert contains an internal SalI site about 860 bp from the EcoRI end. [1] An EcoRI/BglII double digest yields a 2.0 kb fragment containing an LTR and initiator ATG for the gag protein. [1] This was constructed by cloning the 7.7 kb SalI fragment from p60B Sal (containing the SalI fragment of clone 60B) into the SalI site of pSW210 (pBR322 containing the AvrII/SalI fragment of pSNV-60B), thus generating an insert with 2 LTRs. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pSW210 from pBR322 and pSNV-60B, p60B. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
45012
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified