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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pOJ31

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5000
Notes
Medium copy number shuttle vector allowing lacZalpha complementation. Constructed from the cosmid vector pFD304 by addition of a lacZalpha-polylinker fragment and a 2.4 kb PstI-SalI fragment containing the JHJ-3 replicon. The JHJ-3 replicon permits replication in the following actinomycete genera: Streptomyces, Saccharopolyspora and Amycolatopsis. The order of the major features in the plasmid is: JHJ-3 ori - neo - ClaI* - synthetic promoter - pMB1 ori - cos site - lac promoter - SpeI/MCS/XbaI - lacZ. The ClaI* site is methylated, making the ClaI site in the polylinker unique in E. coli dam+ strains. [2] Growth medium: LB plus kanamycin (50 ug/ml)(ATCC number 1236) 37C Deposited by: C.V.Dery Hosts: E.coli, actinomycetes. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77416
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified