Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pOF216
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 12500
- Notes
- Ligating lambda cosR adapters onto the termini of potential inserts and cosL adapters onto the linearized vector enhances the step of circularization of large molecules. [1] The polylinker is inserted in frame within the lacZ gene facilitating selection for blue and white colonies. [1] This is a low copy number mini-F based plasmid vector with rare restriction sites in its polylinker for cloning inserts of up to 100 kb. [1] The depositor recommends adding spectinomycin to the media at a concentration of 20-30 ug/ml at the first establishment of spcR transformants and 15 ug/ml thereafter. (personal communication) The EcoRI site is available for cloning but the lacPO N-terminal region will be deleted. [1] Restriction digests of the clone give the following sizes (kb): BamHI--12.5; HindIII--12.5. (ATCC staff) Medium is -1 1065 plus spectinomycin (15 ug/ml). Hosts: E.coli SC1451, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37765
- GenBank
- X04619
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified