Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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This vector is not available from Addgene.

Plasmid: pNR228

Information

Plasmid Type

Unspecified

Cloning Method

Unknown

Notes

S. pombe vector. ura4+. contains adh-thymidine kinase, allowing counterselection with FuDR (see Kiely et al for the method).

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Unspecified

Sequence

Copy sequence

GTTAACTACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTCTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTACCCCGGTTGATAATCAGAAAAGCCCCAAAAACAGGAAGATTGTATAAGCAAATATTTAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGCCCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTACGTAATACGACTCACTAGTGGGCAGATCTTCGAATGCATCGCGCGCACCGTACGTCTCGAGGAATTCCTGCAGGATCCGGGGATCCCGTGTTTCAGTTAGCCTCCCCCATCTCCCGGGCAAACGTGCGCGCCAGGTCGCAGATCGTCGGTATGGAGCCTGGGGTGGTGACGTGGGTCTGGATCATCCCGGAGGTAAGTTGCAGCAGGGCGTCCCGGCAGCCGGCGGGCGATTGGTCGTAATCCAGGATAAAGACGTGCATGGGACGGAGGCGTTTGGCCAAGACGTCCAAGGCCCAGGCAAACACGTTGTACAGGTCGCCGTTGGGGGCCAGCAACTCGGGGGCCCGAAACAGGGTAAATAACGTGTCCCCGATATGGGGTCGTGGGCCCGCGTTGCTCTGGGGCTCGGCACCCTGGGGCGGCACGGCCGTCCCCGAAAGCTGTCCCCAATCCTCCCGCCACGACCCGCCGCCCTGCAGATACCGCACCGTATTGGCAAGCAGCCCGTAAACGCGGCGAATCGCGGCCAGCATAGCCAGGTCAAGCCGCTCGCCGGGGCGCTGGCGTTTGGCCAGGCGGTCGATGTGTCTGTCCTCCGGAAGGGCCCCCAACACGATGTTTGTGCCGGGCAAGGTCGGCGGGATGAGGGCCACGAACGCCAGCACGGCCTGGGGGGTCATGCTGCCCATAAGGTATCGCGCGGCCGGGTAGCACAGGAGGGCGGCGATGGGATGGCGGTCGAAGATGAGGGTGAGGGCCGGGGGCGGGGCATGTGAGCTCCCAGCCTCCCCCCCGATATGAGGAGCCAGAACGGCGTCGGTCACGGCATAAGGCATGCCCATTGTTATCTGGGCGCTTGTCATTACCACCGCCGCGTCCCCGGCCGATATCTCACCCTGGTCGAGGCGGTGTTGTGTGGTGTAGATGTTCGCGATTGTCTCGGAAGCCCCCAGCACCCGCCAGTAAGTCATCGGCTCGGGTACGTAGACGATATCGTCGCGCGAACCCAGGGCCACCAGCAGTTGCGTGGTGGTGGTTTTCCCCATCCCGTGGGGACCGTCTATATAAACCCGCAGTAGCGTGGGCATTTTCTGCTCCGGGCGGACTTCCGTGGCTTCTTGCTGCCGGCGAGGGCGCAACGCCGTACGTCGGTTGCTATGGCCGCGAGAACGCGCAGCCTGGTCGAACGCAGACGCGTGCTGATGGCCGGGGTACGAAGCCATACGGGATCCTCTAGAGTCGACCTGCAGCAATTCTCTTGCTTAAAGAAAAGAAAAGCGAAGGCACCTGTCCACCACCCACTATTTATACCATGGGAGGCGTCAAACCGAGTTTTTGCGAAAGGATTATCGCACCTCTCCCCAAGTAACATCGGCAAAGCAAAAACGGAGCGGAAGACCAATGAGACGCGGAAGGGGAGCGCCGAATGAGGAAAGCGAGAGAGAAAGAAGTGGATGGAATGATTCCCCGCCAAAGTGGACACATTTCGGGAATCTTCAGGAAGCATGGATCACCTTATGGACAAGAAGATAATCCGGAAGCCAATAGGAGGGCGACAGGGGGTCAGCGATAAGCGTCGGAAAAAACTTTATGATATGGTAAATACGTAGGAGGTTACGGTGCAGAGGAGGGGCCGGTAGCGAGTGATAGCGAGGGAAAGACGATCGCTATCGGGCCGTAAGGAGGAAATGGGAGGTTGGGGGAAGGATGCGTTGGAATGCGGAGTAGAGAAATTGAAGAGAGAGAGAGGACACACCTAGAGAGAAAGAAATGGATATAGAGAAAGAGAAAAAATAAGAGGAATACGGATACGATGGAGTGCGTTGAATGAGAGAAAAGAAAAGAAAAGGAATGATAAGAGAAGGAATGAAAATTGTTTTGTTTTGTTTTCAATTGCTTTCTTTTGTTTTCTTTGTCTTTGTTCTTCCTTCCCTTGTCCCGATGCTGTTCTTCTCACCAGCTTCCGCATGATAGCCATTTTCTTAGTTGTTGTAGGGCATGCAAGCTTAGCTACAAATCCCACTGGCTATATGTATGCATTTGTGTTAAAAAAGTTTGTATAGATTATTTAATCTACTCAGCATTCTTTCTCTAAATAGGAATTTGTTACTTAATGGAGAAAAAAATGTTTCGATTTACCTAGTGTATTTGTTTGTATACTCACGTTTAATTTCAAACATCCATTCTATCTTGTGTAATTTTTGGCATGGTGAAAAAGATAATCAGCCTTATAATCTTTACAAAAGTAAGAAATTCTGTAAATAAGCCTTAATGCCCTTGCTTTAAATTAAAATGGTTCTTTTTCATGATAATGTTTGCACTTTGTGAATATATTTTAGATAGTTCTGTGAGGTATAATTAAGATGTTTTAGAGACTTATACAATTTTGTCTTTATAAATTCTTAATTGATTTTACCATCCCAGTTTAACTATGCTTCGTCGGCATCTCTGCACATGTCGTGTTTTCTTACCGTATTGTCCTACCAAGAACCTCTTTTTTGCTTGGATCGAAATTAAAGGTTTAAAAGCAAAGTTATGGATGCTAGAGTATTTCAAAGCTATTCAGCTAGAGCTGAGGGGATGAAAAATCCCATTGCCAAGGAATTGTTGGCTTTGATGGAAGAAAAGCAAAGCAACTTGTCAGTCGCGGTCGATTTGACGAAGAAATCCGAAATCTTAGAATTGGTAGATAAAATTGGACCCTATGTCTGTGTTATCAAGACACATATTGACGTTGTCGAGGATTTCGACCAGGATATGGTAGAAAAACTGGTGGCCTTAGGTAAAAAGCATCGTTTTCTTATCTTTGAGGATCGCAAATTCGCAGACATTGGAAATACCGTCAAGCTACAATATGCATCTGGTGTGTACAAAATTGCTTCTTGGGCTCATATCACAAATTGCCATACAGTGCCAGGCGAGGGTATTATACAAGGCCTCAAAGAAGTTGGTTTACCTTTGGGACGTGGTCTCTTGCTTTTGGCTGAAATGTCTTCCAAAGGCTCTTTGGCTACTGGTTCCTACACAGAGAAAACCTTAGAATGGTTTGAGAAGCATACCGATTTTTGCTTTGGCTTTATAGCTGGTCGTCGATTTCCTAACCTTCAAAGCGACTACATAACTATGTCCCCTGGTATCGGCTTGGATGTTAAAGGAGACGGGCTGGGACAGCAATATCGTACTCCTGAAGAAGTGATTGTAAACTGCGGTAGCGATATCATCATTGTTGGTCGTGGAGTCTATGGAGCTGGTCGTAATCCTGTTGTCGAAGCCAAGAGATATAGAGAAGCTGGTTGGAAGGCATATCAGCAAAGACTTTCTCAGCATTAAAAAAAGACTAATGTAAAATTTTTTTGGTTGGTTATTGAAAAAGTCGATGCCTTGTTTGCGTTTGTTTTCCTAGGCGTTTTATGTCAGAAGGCATTTAGAATTAGTATACAAGTACTCTTTGGTAAAATTTTATGTAGCGACTAAAATATTAACTATTATAGATAAACACCTTGGGAATAAAAAGTAATTTGCTATAGTAATTTATTAAACATGCTCCTACAACATTACCACAATCTTTTCTCTTGGATTGACATTGAATAAGAAAAGAGTGAATTTTTTTAGACTTGTAATGATAACTATGTACAAAGCCAATGAAAGATGTATGTAGATGAATGTAAAATACCATGTAGACAAACAAGATAAAACTTGGTTATAAACATTGGTGTTGGAACAGAATAAATTAGATGTCAAAAAGTTTCGTCAATATCACAAGCTTCCCATGGTGACGTCACCGGTTCTAGATACCTAGGTAGGCATTTTGTTTAGTTAAAGGTACTTAAGTAATTAGTATAAACGAACCAATTTTATAATCAGGAAGTTAAGTGAATGGTAGCACATGTCGTAAAAATTGTGAATTTTTATTGAATAATATTTTAAATACAAGCCTTTCTAGACTAGGTATACTCATAAACATATGAGCAAAAGGATAGAGGAGATTACATTGCATCTTCTACAAATTTATTTATTGCCCTTTACTGAAAAATTAAATAATGAGTACTAAATGATAAAAAGCGCTCAGTACAAGAAAGATTGCAAAAATATTGCATTCTTCATGAATTAAAGTTGCATATAAGGCATATTGAAAGTAATAGTACTAAAACAGCAGTTAGCGAAAATTAATAGAATTATATTCGCAAGACAATTGTGACATTAAATTAAAAAATTGTAGAATTTTTACTATCCTCTTTAACGCCATGAGCCTTTATAAAAAGGTTAAATTAGTTTTAACATTCTTTTTTTGAGTAAGAGTTTACAATTTATCAAAACCTGTGTTATTATATTCATTAGTTTCAATTTATTAGCATCTAGAGAAAAATCAATTGGCAGTTACATTGTTGGAATTTATGAAGAAAAAGAATCTACAACGGAGAATAAGTTGCTGATCGCTTTCCCTAAAATTGTATATTTTGCTGAGCTTATTTTGACATTTCGTTGAAGTTTTCTCTAATTCGCATTCATTTTAAGTAAAACAATGAGAAATAAAATTACAAAAAATACAAATTAAAATACAATTTTTAGCTATAATTATAGACCTAGGTGAGCTCTGGTACCCTCTAGTCAAGGCCTTAAGTGAGTCGTATTACGGACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTCGCTTGGTAATAAAGCCCGCTTCGGCGGGCTTTTTTTT

Published Plasmid Map