Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pNN414
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 15809
- Notes
- Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI--15.9; XhoI--11.0, 3.4, 1.0; (ATCC staff) BamHI--14.0, 1.85; BamHI/EcoRI--8.2, 5.8, 1.85; SalI/BamHI--7.4, 6.6, 1.85. (ATCC staff) XhoI linkers were ligated to the blunt ended 4.2 kb PvuI/BamHI fragment of pSV2neo (with neomycin resistance and ColE1 origin). This was then cloned into the SalI site of pYAC4. Contains telomer elements from Tetrahymena. To facilitate chromosome walking, the ends of the insert can be recovered in E.coli. The artificial chromosomal DNA can be digested with XhoI, ligated, and used to transform E.coli to amp or kan resistance. Cloning into the EcoRI site insertionally inactivates SUP4. G418 resistance is expressed in mammalian cell lines to permit genetic complementation experiments. Constructed by Christopher Traver. Medium is 1227 LB plus ampicillin. Hosts: E.coli DH1, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae AB1380. Related vectors: pSV2-neo, pYAC4. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37682
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified