Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pNH218
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 11500
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 3.8; HindIII--6.8, 4.7. (ATCC staff) A 1.1 kb EcoRI fragment with Bacillus licheniformis alpha-amylase regulatory & signal sequences was cloned into pUC9 cut with EcoRI/SmaI. A 3.0 kb BamHI/HindIII fragment (E.coli phoA minus signal sequences and the first 5-13 aa) was inserted. pBs81/6 (Bacillus replication) was cut with HindIII and cloned into the HindIII site. An E.coli/Bacillus spp. shuttle expression vector for the construction and secretion of fusion proteins. Contains the alkaline phosphatase gene of E.coli. The plasmid appears to be unstable. We recommend that the ATCC-provided stock be streaked for single colonies and that a colony with the correct restriction pattern be selected. (ATCC staff) Medium is 1065 LB medium. Hosts: Bacillus subtilis 168, Bacillus subtilis, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 53063
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified