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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pNF2

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
7800
Notes
The spcR marker is expressed in R. capsulatus. A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia. A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter. Restriction digests of the clone give the following sizes (kb): SalI--8.2; BamHI--8.2; EcoRI/PstI--4.2, 3.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, Rhodobacter. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37615
GenBank
M29400, M29401, M29402
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified