Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pND201
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4013
- Notes
- Because cI857 is expressed by the plasmid itself, from its natural promoter PM, the vector may be used in virtually any E.coli strain. Constructed by inserting an oligonucleotide containing a HpaI site and a ribosome-binding site between the BamHI and SmaI sites of pCE30. Potential inserts should be trimmed with Bal31 exonuclease to remove 5' sequences to within 10 bp of the start codon, and blunt-end ligated into the HpaI site downstream of a strong ribosome-binding site. The sequence surrounding the cloning sites has been used to design primers for direct sequencing, including the M13 universal primer. Restriction digests of the clone give the following sizes (kb): BamHI--4.0; BamHI/EcoRI--4.0; BglI--2.9, 1.15. (ATCC staff) Expression vector containing a strong ribosome-binding site and primer sites useful for sequencing. Encodes cI857. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli AN1459, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37831
- GenBank
- M36426
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified