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Vector Database


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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pMX-EGFP-gw-IP

Information

Plasmid Type
Mammalian Expression, Retroviral
Cloning Method
Unknown
5' Sequencing 1 Primer
pBMN5'
Bacterial Resistance
Ampicillin
Selectable Marker
Puromycin
Notes
For constructing pMX-EGFP-gw-IP, a gateway cassette rfB (Invitrogen) was introduced into XhoI (blunted) site of pEGFP-C2 (Clontech). We named this product, pEGFP-gw. Second, an EcoRV/XbaI (blunted) fragment of pIRESpuro (Clontech) was introduced into BamHI (blunted)/SalI (blunted) site of pMX. Finally, an Eco47III (blunted)/HindIII (blunted) fragment of pEGFP-gw was introduced into the EcoRI (blunted)/BamHI (blunted) of pMX-IP vector. This product is pMX-EGFP-gw. Information provided by Dr. Kazutoshi Takahashi and Dr. Shinya Yamanaka.
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Retroviral