Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMX-EGFP-gw-IP
Information
- Plasmid Type
- Mammalian Expression, Retroviral
- Cloning Method
- Unknown
- 5' Sequencing 1 Primer
- pBMN5'
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Puromycin
- Notes
- For constructing pMX-EGFP-gw-IP, a gateway cassette rfB (Invitrogen) was introduced into XhoI (blunted) site of pEGFP-C2 (Clontech). We named this product, pEGFP-gw. Second, an EcoRV/XbaI (blunted) fragment of pIRESpuro (Clontech) was introduced into BamHI (blunted)/SalI (blunted) site of pMX. Finally, an Eco47III (blunted)/HindIII (blunted) fragment of pEGFP-gw was introduced into the EcoRI (blunted)/BamHI (blunted) of pMX-IP vector. This product is pMX-EGFP-gw. Information provided by Dr. Kazutoshi Takahashi and Dr. Shinya Yamanaka.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Retroviral