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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pMMB278

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8990
Notes
Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. [1] Constructed by replacing the MluI/EcoRI fragment of pMMB190 (taclacUV5 promoter) with the MluI/EcoRI fragment of pMMB311 (tac promoter). [2] Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; EcoRV--5.4, 3.7; SspI--5.0, 1.7, 1.5, 0.9; EcoRI/MluI--8.2, 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 209093 Hosts: E.coli TB1, broad host range, E.coli, E.coli DH5alphaF'. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37812
GenBank
M37849
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified