Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMMB277
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9237
- Notes
- Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter). Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml). NCBI gi: 209093 Hosts: E.coli TB1, broad host range, E.coli, E.coli DH5alphaF'. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37811
- GenBank
- M37849
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified