Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMMB208
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9089
- Notes
- Created by Moore, July 1995, under contract with NCBI. pMMB207 (ATCC 37809) and pMMB208 (ATCC 37810) differ in the orientation of the multiple cloning site with pMMB207 having the EcoRI site near the promoter and pMMB208 having the HindIII site nearer. Does not have a start codon at a suitable distance after the ribosome-binding site, so translation will be efficient only if the insert contains an RBS followed appropriately by a start codon. Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.9; SspI--4.1, 1.8, 1.6, 1.0, 0.7; EcoRI/MluI--8.3, 0.8. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml). Hosts: E.coli TB1, broad host range, E.coli, E.coli DH5alphaF'. Related vectors: pMMB67HE, pMMB206. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37810
- GenBank
- M37848
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified