Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMB
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5590
- Notes
- Deposited by: J. Justin McCormick The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. [1] Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ. [1] Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by an RSV enhancer-MMTVLTR promoter at one end and splicing and polyadenylation signals at the other. [1] The plasmid contains the following restriction sites (bp from position 0): NotI--0; EcoRI--9, 3071; ClaI--425, 1560, 1745; Drd--1398, 3583, 5452; PvuI--1596, 2953, 4741; BglII--1782; SphI--2907, 3110; NsiI--2909; HindIII--99, 1794, 3112; BsaI--4441. (personal communication) The order of the major features in this plasmid is: NotI - 5' RSV enhancer - MMTV LTR 3' - ClaI - 5' lacZ'amb5 - NruI/MCSII''/HindIII - 3' lacZ'amb5 - splicing - polyadenylation - NsiI - 3' lacZ' EcoRI/MCS/HindIII - 5' lacZ'- pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--2.5, 1.8, 1.3; EcoRI--3.0, 2.6; HindIII--2.6, 1.7, 1.3; BglII--5.6; ClaI--4.3, 1.3. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM105, E.coli JM109, vertebrate cells. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77385
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified