Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMAMPF3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4354
- Notes
- Expression vector permitting production of a fusion protein and containing signal sequences for protein export. Also permits production of ssDNA and contains priming sites useful for sequencing. Encodes the ompA leader. Contains a chloramphenical acetyltransferase gene with an amber mutation useful for gapped-duplex mutagenesis experiments. Expression can be induced by a temperature shift in hosts providing a temperature-sensitive lambda repressor, or by batch transduction in a growing culture to repressor-negative hosts. The order of the major features in this phagemid is:lambdaPL promoter - ompA leader - NaeI site - MCS - priming site - fd ori - pMB1 ori - bla - cat (am). Restriction digests of the clone give the following sizes (kb): SalI--4.3; PstI--2.3, 2.0; XbaI--4.3; EcoRI--4.3; HindIII--4.3. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli. Related vectors: pBR325, pMAC5-8. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77132
- GenBank
- M33637
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified