Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pLUC
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5411
- Notes
- Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. Differs from pLUCS (ATCC 77415) only in the polylinker. Constructed from pSVOALdelta5' by inserting a 2.6 kb BamHI fragment, containing the firefly (Photinus pyralis) luciferase gene and SV40 sequences into the BamHI site of pBluescriptSK+, followed by the removal of the 3' BamHI site. Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C. The order of the major features in the phagemid is: 3' lacZ - XhoI/MCS/BamHI - 5' luc 3' - SV40 small t intron - SV40 polyadenylation signal - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori. [1] Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: A.D.Ward Hosts: E.coli, vertebrate. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77414
- GenBank
- U03687
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified