Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pLS101
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6400
- Notes
- Cloning into the unique KpnI and SacI sites inactivates malM. Cloning into the 4.2 kb vector fragment resulting from HindIII digestion removes malM. Mal- recombinants can be enriched by penicillin selection of tetR bacteria. Cloning into the unique HpaII site inactivates tet. Removal of the smaller BclI fragment also inactivates tet. EcoRI digestion removes a dispensible EcoRI fragment. pLS101 contains the following restriction sites (kb markers according to the published map): PstI--0/6.4, 0.8; BclI--1.8, 2.8; HpaII--2.4; EcoRI--3.0, 4.1; HindIII--4.2, 4.9, 5.1, 5.3, 5.5, 6.2; KpnI--4.75; SacI--5.6. Strain is noncapsulated and reportedly never reverts to encapsulated form. This was constructed from pLS69 (ATCC 39937) by ligating a partial 2.0 kb HindIII fragment containing malM to a 4.4 kb HindIII from complete digest of pLS69. The latter fragment contains tet as well as the origin of replication. Medium is 1420 260 plus sucrose (0.2%) plus tetracycline (0.5 mcg/ml). Hosts: Streptococcus pneumoniae R6, broad host range, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39938
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified