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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLKC482

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9900
Notes
Restriction digests of the clone give the following sizes (kb): EcoRI--10.2, BamHI--10.2, PstI--6.2, 4.4, HindIII--10.2. (ATCC staff) One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the E.coli genome. (personal communication) A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid. The 6.3 kb lacZY-kanR cassette is released with SmaI. Medium is 1227 LB plus ampicillin. Hosts: E.coli TX528, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37596
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified