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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLKC480

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9900
Notes
The 6.3 kb lacZY-kanR cassette is released with SmaI. A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid. One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the E.coli genome. Restriction digests of the clone give the following sizes (kb): EcoRI--10.05; BamHI--10.05; PstI--6.0, 4.4; HindIII--10.05. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli TX528, E.coli. Related vectors: pNEO, pNM480, pLKC481, pLKC482. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37594
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified