Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pKM1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4378
- Notes
- Assembled from pKO1 and phage lambda by F. Pfeiffer. This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galKgene. Also, a polylinker was cloned before this gene. In addition, the lambda tr1 transcription terminator was inserted BEFORE the galK gene to allow measurement of very strong promoters due to read-through transcription. Promoter-cloning vector for isolating and characterizing very strong promoters. [1] This vector was constructed by inserting a rho-dependent terminator (lambdaTRI) into the SmaI site of pKO1 (ATCC 37126). This reduces the number of transcripts reaching the galK coding sequences to 30% or less & is thus useful for strong promoters. [2] Medium is 1227 LB plus ampicillin. Hosts: E.coli N100, E.coli. Related vectors: EcoGalK, pBR322, pKO-1, lambda. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37285
- GenBank
- L08921
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified