Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pKL19-2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 2675
- Notes
- Created by Moore, July 1995, under contract with NCBI. Expression vector permitting production of a fusion protein and visual detection of recombinants, using the beta-galactosidase alpha peptide as the reporter group. (ATCC staff) A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated d[pCCCCGGGG]. (personal communication) Distributed in aliquots of 2 ug (100 ng/ul). (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: depositor-supplied DNA, E.coli. Related vectors: pUC19. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 67997
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified