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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pKBY2

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9900
Notes
The BamHI site was removed from trpC by sodium bisulfite mutagenesis. A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with wild-type Aspergillus nidulans trpC was ligated into the SalI, and a BamHI linker into the NruI site of pBR329. This is a cosmid vector for isolating genes by complementation of mutations in Aspergillus nidulans. It can also be used for library construction. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
39898
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified