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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pK184

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
2432
Notes
A general purpose plasmid vector for use in complementation analysis with vectors having a ColE1 or pMB1 origin of replication. pK184 consists of bases 2092-2661/0-1177 of pK18 and bases 1520-836 of pACYC184. There are 2 XbaI sites in the plasmid. pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning sites in opposite orientations, interrupting the lacZalpha coding sequence. Constructed by M. G. Jobling. (personal communication) Restriction digests of the clone give the following sizes (kb): HindIII--2.4; BamHI--2.4; EcoRI--2.4; XbaI--1.45, 0.95. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli XL1-Blue, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37766
GenBank
U00800
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence