Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pJS97
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7200
- Notes
- ATCC size is 7300 bp. pJS97 has URA3 marker and pJS98 has TRP1 marker; double selection ensures only clones with a whole YAC. YL-type shuttle vector useful for construction of genomic libraries. Contains promoters for in vitro RNA synthesis and permits recovery of end fragments as plasmids in E. coli. [4] pJS97 (ATCC 77153) and pJS98 (ATCC 77154) are used together to construct artificial chromosomes. Each plasmid is digested with ClaI and the cloning enzyme. [4] The order of the major features in this plasmid is: ClaI/MCS/EcoRI - T7 promoter - ARSH4 - CEN4 - URA3 - SUP11 - pMB1 ori - bla - SacI - BamHI - SphI - TEL. [4] To recover both ends of a YAC insert as plasmid, DNA of a YAC-containing strain is digested to completion with BamHI, SphI or SacI, diluted, ligated, and transformed into E. coli (to ampicillin resistance). [4] The insert of a resultant plasmid (which includes the T7 promoter) extends from an end of the YAC insert to the first site of the enzyme used for digestion. [4] Restriction digests of the clone give the following sizes (kb): NotI--7.4; BglII--7.4; SalI--7.4; EcoRI--7.4; SacI--7.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: S.cerevisiae YPH274, E.coli HB101, E.coli, S.cerevisiae, S.cerevisiae YPH252, S.cerevisiae YPH857. Related vectors: pUC19, pJS98. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77153
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified