Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pJA70
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5078
- Notes
- YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system. (personal communication) The order of the major features in this plasmid is:lacZ'/MCS - CEN6 - ARS H4 - HIS3 - kanR - pMB1 ori. (personal communication) Restriction digests of the clone give the following sizes (kb): PstI--2.2, 1.8, 1.2; HindIII--3.1, 1.9, 0.2; KpnI--2.9, 2.1; EcoRI--5.1. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77258
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified