Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pJ5Eomega
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3700
- Notes
- Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7. (ATCC staff) Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation A site), and MMTV LTR (-360 to +150, glucocorticoid regulatory sequences, cap site) and MSV enhancer. (personal communication) Demonstrates expression inducible by dexamethasone. A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the MMTV LTR promoter and MSV enhancer. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli HB101, vertebrate cells, E.coli, E.coli DH5. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37722
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified