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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pIA1

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8800
Notes
YE-type shuttle vector permitting FLP-driven high copy number. (personal communication) In S. cerevisiae, requires a host expressing FLP such as YPH485 or YPH494, or a host modified by pFV17 (ATCC 77170). (personal communication) When propagated in a strain carrying an integrated copy of GAL10-FLP, the copy number is 10-20 without induction and 200-400 when induced by galactose. The order of the major features in this plasmid is:REP3 - D - REP1 - REP2 - kanR - MCS (NotI - EcoRI/NotI) - URA3. Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle (interrupting FLP) into the SmaI site of pHSS6 which had been modified by the addition of a URA3 gene. Restriction digests of the clone give the following sizes (kb): BamHI--8.8; HindIII--4.3, 3.3, 1.2; NotI--8.8; ClaI--8.8. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH485, Saccharomyces cerevisiae YPH494. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77159
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified